CRMAGE: CRISPR Optimized MAGE Recombineering
نویسندگان
چکیده
منابع مشابه
CRMAGE: CRISPR Optimized MAGE Recombineering
A bottleneck in metabolic engineering and systems biology approaches is the lack of efficient genome engineering technologies. Here, we combine CRISPR/Cas9 and λ Red recombineering based MAGE technology (CRMAGE) to create a highly efficient and fast method for genome engineering of Escherichia coli. Using CRMAGE, the recombineering efficiency was between 96.5% and 99.7% for gene recoding of thr...
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Escherichia coli is one of the most widely used hosts for recombinant protein production. In most cases, especially when high levels of target proteins are required, plasmid-borne expression is the method of choice for this purpose. The availability of a plethora of E. coli plasmid expression vectors with various promoters, selection markers, purification tags, fusion partners and auxiliary pro...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2016
ISSN: 2045-2322
DOI: 10.1038/srep19452